Search Repository Universitas Fort De Kock

Penelitian ini bertujuan mengkarakterisasi senyawa bioaktif dan mengevaluasi
aktivitas antikanker ekstrak etanol kulit kayu Bajakah (Spatholobus littoralis
Hassk.) yang berasal dari dataran tinggi Kabupaten Lima Puluh Kota. Ekstrak
diperoleh melalui metode maserasi menggunakan etanol 96%. Uji toksisitas awal
dilakukan dengan metode Brine Shrimp Lethality Test (BSLT), sedangkan aktivitas
sitotoksik terhadap sel kanker payudara MCF-7 diuji dengan metode WST-8
menggunakan cisplatin sebagai kontrol positif. Analisis LC-HRMS
mengidentifikasi adanya senyawa yang diketahui memiliki potensi sebagai
antikanker, di antaranya Biochanin A, Pelargonidin, 5,6,2-Trimethoxyflavone,
Formononetin, Alpinetin, 4,2,4-Trihydroxy-3-methoxydihydrochalcone, dan
Betaine. Hasil BSLT menunjukkan nilai LC50 sebesar 501,53 mg/L (kategori
toksik), sedangkan uji WST-8 menghasilkan IC50 sebesar 23.2167 g/mL, yang
memenuhi kriteria potensi sitotoksik menurut NCI (

This study aimed to characterize bioactive compounds and evaluate the anticancer
activity of the ethanol extract of Bajakah bark (Spatholobus littoralis Hassk.),
collected from the highlands of Lima Puluh Kota Regency. The extract was
obtained through maceration using 96% ethanol. Initial toxicity testing was
conducted using the Brine Shrimp Lethality Test (BSLT), while cytotoxic activity
against MCF-7 breast cancer cells was assessed using the WST-8 assay, with
cisplatin as the positive control. LC-HRMS analysis identified several compounds
known for their anticancer potential, including: Biochanin A, Pelargonidin, 5,6,2-
Trimethoxyflavone, Formononetin, Alpinetin, 4,2,4-Trihydroxy-3-
methoxydihydrochalcone, and Betaine. The BSLT results showed an LC50 value of
501.53 mg/L, categorized as toxic, while the WST-8 assay yielded an IC50 value of
23.2167 g/mL, which meets the National Cancer Institute (NCI) criteria for
cytotoxic potential (IC50 < 30 g/mL). These findings suggest that the ethanol
extract of Bajakah bark holds promise as a natural therapeutic agent for the
treatment of breast cancer.