Search Repository Universitas Fort De Kock

Tanaman gambir (Uncaria gambir (W.Hunter). Roxb) memiliki potensi besar
dalam pengobatan berbagai infeksi bakteri. Penelitian ini bertujuan untuk
mengetahui aktivitas antibakteri terhadap Escherichia coli. Metode penelitian
meliputi pembuatan ekstrak dan fraksi daun gambir dengan beberapa pelarut,
diikuti dengan uji skrining fitokimia dan analisis LC-HRMS. Uji antibakteri
dilakukan menggunakan metode difusi cakram pada media Mueller Hinton Agar
(MHA) dengan parameter berupa pengukuran diameter zona hambat.
Konsentrasi ekstrak yang digunakan adalah 25%, 50%, dan 75%, dengan
kloramfenikol sebagai kontrol positif dan DMSO sebagai kontrol negatif. Hasil
penelitian menunjukkan ekstrak etanol menghasilkan zona hambat 16,2822,26
mm (kuatsangat kuat), sedangkan ekstrak air tidak menunjukkan aktivitas.
Fraksi air memberikan daya hambat tertinggi 22,6026,13 mm (sangat kuat),
diikuti fraksi etil asetat 14,5221,31 mm (kuatsangat kuat), dan fraksi n-heksan
12,1017,83 mm (sedangkuat). Data ini menunjukkan bahwa ekstrak etanol,
fraksi etil asetat, fraksi air, dan fraksi n-heksan daun gambir memiliki potensi
signifikan dalam menghambat pertumbuhan Escherichia coli.

The gambir plant (Uncaria gambir (W. Hunter) Roxb.) has significant potential in
the treatment of various bacterial infections. This study aimed to evaluate its
antibacterial activity against Escherichia coli. The research involved the
preparation of gambir leaf extracts and their fractions using various solvents,
followed by phytochemical screening and LC-HRMS analysis. Antibacterial
activity was assessed using the disk diffusion method on Mueller Hinton Agar
(MHA), with the inhibition zone diameter as the main parameter. Extract
concentrations tested were 25%, 50%, and 75%, with chloramphenicol as a
positive control and DMSO as a negative control. The results showed that the
ethanol extract produced inhibition zones ranging from 16.28 to 22.26 mm
(indicating strong to very strong activity), whereas the water extract showed no
antibacterial activity. Among the fractions tested, the water fraction exhibited the
highest inhibitory activity, with inhibition zones of 22.6026.13 mm (very
strong), followed by the ethyl acetate fraction with zones of 14.5221.31 mm
(strong to very strong), and the n-hexane fraction with 12.1017.83 mm (moderate
to strong). These findings demonstrate that the ethanol extract, as well as the
water, ethyl acetate, and n-hexane fractions of gambir leaves, possess significant
antibacterial potential against Escherichia coli.