Search Repository Universitas Fort De Kock

Cluster of differentiation-8 (CD8) merupakan marker dari sel T sitotoksik.
Sel CD8 sangat penting terhadap penanganan penyakit infeksi virus, kanker/
tumor dan bakteri intraseluler, dan upaya untuk meningkatkan sistem pertahanan
tubuh. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian fraksi etil
asetat daun alpukat (Persea americana Mill.) terhadap aktivitas sel CD8 sebagai
imunomodulator pada tikus putih jantan. Latar belakang penelitian ini didasari
oleh pentingnya peningkatan sistem imun. Daun alpukat diketahui mengandung
senyawa bioaktif seperti flavonoid, tannin, saponin dan triterpenoid yang
berpotensi meningkatkan sistem imun. Penelitian ini menggunakan desain true
experimental dengan rancangan pre-post. Tikus dibagi menjadi 3 kelompok, yaitu
kelompok kontrol negatif, kelompok pembanding (Stimuno Forte), dan kelompok
perlakuan (fraksi etil asetat daun alpukat) dosis 2 ml/200 gr BB yang diberikan
secara oral selama 14 hari. Pengukuran kadar CD8 dilakukan dengan metode
ELISA sebelum dan sesudah perlakuan. Analisis data menggunakan One-Way
ANOVA menunjukkan adanya perbedaan signifikan kadar CD8 antar kelompok
(p

Cluster of Differentiation 8 (CD8) is a marker of cytotoxic T cells. CD8 cells
play a crucial role in combating viral infections, cancer/tumors, and intracellular
bacterial infections, as well as in efforts to strengthen the bodys immune system.
This study aims to determine the effect of the ethyl acetate fraction of avocado
leaves (Persea americana Mill.) on CD8 cell activity as an immunomodulator in
male white rats. The background of this research is based on the importance of
enhancing the immune system. Avocado leaves are known to contain bioactive
compounds such as flavonoids, tannins, saponins, and triterpenoids, which have
the potential to boost immunity. This study used a true experimental design with a
pre-post design. The rats were divided into three groups: a negative control group,
a comparison group (Stimuno Forte), and a treatment group (ethyl acetate fraction
of avocado leaves) with a dose of 2 ml/200 g body weight, administered orally for
14 days. CD8 levels were measured using the ELISA method before and after
treatment. Data analysis using One-Way ANOVA showed a significant difference
in CD8 levels among the groups (p < 0.05), where the treatment group showed an
increase of 20 cells/l, comparable to the comparison group with an increase of
22.02 cells/l. It can be concluded that the ethyl acetate fraction of avocado leaves
has potential as a natural immunomodulator through the enhancement of CD8 cell
activity.