Search Repository Universitas Fort De Kock

Nyeri merupakan respon fisiologis akibat adanya rangsangan yang merusak jaringan
dan membutuhkan penanganan farmakologis maupun non-farmakologis. Salah satu alternatif
pengembangan obat analgetik berbahan alam adalah melalui pemanfaatan daun kirinyuh
(Chromolaena odorata) dan umbi bawang dayak (Eleutherine bulbosa). Kedua tanaman
tersebut mengandung senyawa metabolit sekunder seperti flavonoid, alkaloid, dan saponin
yang berpotensi memberikan efek analgetik. Penelitian ini bertujuan untuk mengetahui
aktivitas analgetik infusa daun kirinyuh, umbi bawang dayak, serta kombinasi keduanya

terhadap mencit putih jantan dengan menggunakan metode writhing test. Simplisia masing-
masing ditimbang sebanyak 25 gram, dimasukkan ke dalam beaker glass 500 mL, lalu

ditambahkan 100 mL aquades. Perebusan dilakukan pada suhu 90C selama 15 menit dengan
sesekali pengadukan. Kombinasi infusa dibuat dengan perbandingan 1:1 (12,5 gram : 12,5
gram). Hewan uji dibagi ke dalam 6 kelompok, yaitu kontrol negatif (Na-CMC 0,5%), kontrol
positif (asam mefenamat 500 mg, 0,2 mL), kontrol pembanding, infusa daun kirinyuh, infusa
umbi bawang dayak, dan kombinasi infusa. Amati jumlah geliat yang muncul setiap 5 menit
selama 30 menit setelah induksi nyeri dengan asam asetat. Hasil penelitian menunjukkan
bahwa infusa daun kirinyuh, umbi bawang dayak, maupun kombinasi keduanya mampu
menurunkan jumlah geliat mencit dibandingkan kontrol negatif. Kombinasi infusa memberikan
aktivitas analgetik yang lebih tinggi dibandingkan pemberian tunggal, namun masih lebih
rendah dibandingkan dengan kontrol positif. Dapat disimpulkan bahwa infusa kombinasi daun
kirinyuh (Chromolaena odorata) dan umbi bawang dayak (Eleutherine bulbosa) memiliki
aktivitas analgetik pada mencit putih jantan, sehingga berpotensi dikembangkan sebagai
kandidat obat herbal analgetik.

Pain is a physiological response resulting from stimuli that damage tissue and requires
both pharmacological and non-pharmacological management. One alternative for developing
natural analgesic drugs is through the use of kirinyuh leaves (Chromolaena odorata) and dayak
onion bulbs (Eleutherine bulbosa). Both plants contain secondary metabolites such as
flavonoids, alkaloids, and saponins, which have potential analgesic effects. This study aimed
to determine the analgesic activity of the infusions of kirinyuh leaves, dayak onion bulbs, and
their combination in male white mice using the writhing test method. Each simplicia sample
was weighed 25 grams and placed into a 500 mL beaker glass, then 100 mL of distilled water
was added. The mixture was boiled at 90C for 15 minutes with occasional stirring. The
combined infusion was prepared at a 1:1 ratio (12.5 g : 12.5 g). The test animals were divided
into six groups: negative control (0.5% Na-CMC), positive control (mefenamic acid 500 mg,
0.2 mL), comparative control, kirinyuh leaf infusion, dayak onion infusion, and the combined
infusion. The number of writhing responses was observed every 5 minutes for 30 minutes after
pain induction using acetic acid. The results showed that the infusions of kirinyuh leaves, dayak
onion bulbs, and their combination reduced the number of writhing responses in mice
compared to the negative control. The combined infusion demonstrated higher analgesic
activity compared to single infusions, although still lower than the positive control. It can be
concluded that the infusion combination of kirinyuh leaves (Chromolaena odorata) and dayak
onion bulbs (Eleutherine bulbosa) exhibits analgesic activity in male white mice and has
potential to be developed as a herbal analgesic candidate.