Search Repository Universitas Fort De Kock

Kanker payudara merupakan salah satu kanker dengan prevalensi tertinggi. Terapi
konvensional seperti kemoterapi sering menimbulkan efek samping pada sel sehat.
Penelitian ini bertujuan mengevaluasi aktivitas antikanker ekstrak non polar daun
piladang hijau (Coleus monostachyus) terhadap sel kanker MCF-7 secara in vitro
serta menentukan pelarut non polar paling efektif dalam mengekstraksi senyawa
sitotoksik. Ekstraksi dengan metode maserasi menggunakan pelarut kloroform,
diklorometana, dan n-heksan. Kloroform menghasilkan rendemen tertinggi
(18,5%), diikuti diklorometana (17,5%) dan n-heksan (15,5%). Skrining fitokimia
menunjukkan seluruh ekstrak mengandung alkaloid, flavonoid, tanin, dan steroid.
Uji toksisitas metode Brine Shrimp Lethality Test (BSLT) menunjukkan nilai LC50
terendah pada ekstrak kloroform (100,50 g/mL), diikuti n-heksan (143,05 g/mL)
dan diklorometana (240,71 g/mL), semuanya tergolong toksik sedang. Uji
sitotoksik metode MTT Assay juga menunjukkan IC50 terendah pada ekstrak
kloroform (128,82 g/mL), diikuti n-heksan (150,02 g/mL) dan diklorometana

(278,84 g/mL). Seluruh ekstrak menunjukkan efek sitotoksik secara konsentrasi-
dependen. Hasil menunjukkan bahwa ekstrak daun Coleus monostachyus dengan
pelarut kloroform memiliki potensi tertinggi sebagai agen antikanker alami.
Penelitian ini mendukung pemanfaatan tanaman obat sebagai alternatif terapi
kanker yang lebih aman dan efektif.

Breast cancer is one of the most prevalent types of cancer globally. Conventional
treatments such as chemotherapy often cause adverse side effects on healthy cells.
This study aimed to evaluate the anticancer activity of non-polar solvent extracts
from green piladang leaves (Coleus monostachyus) against MCF-7 breast cancer
cells in vitro, and to determine the most effective non-polar solvent for extracting
cytotoxic compounds. Extraction was carried out using the maceration method with
three non-polar solvents: chloroform, dichloromethane, and n-hexane. Chloroform
yielded the highest extract percentage (18.5%), followed by dichloromethane
(17.5%) and n-hexane (15.5%). Phytochemical screening revealed that all extracts
contained alkaloids, flavonoids, tannins, and steroids. Toxicity testing using the
Brine Shrimp Lethality Test (BSLT) showed the lowest LC50 value in the chloroform
extract (100.50 g/mL), followed by n-hexane (143.05 g/mL), and
dichloromethane (240.71 g/mL)all classified as moderately toxic. Cytotoxicity
testing via the MTT Assay also showed the lowest IC50 in the chloroform extract
(128.82 g/mL), followed by n-hexane (150.02 g/mL) and dichloromethane
(278.84 g/mL). These results indicate that the chloroform extract of Coleus
monostachyus leaves possesses the strongest potential as a natural anticancer
agent. This research supports the potential use of medicinal plants as safer and
more effective alternative therapies for cancer treatment.