Search Repository Universitas Fort De Kock

Perkembangan nanoteknologi telah membawa nanopartikel perak (AgNPs), dengan
ukuran 1-100 nm, ke garis depan penelitian medis. AgNPs diketahui dapat
menyebabkan kematian sel kanker melalui stres oksidatif. Penelitian ini bertujuan

untuk mensintesis dan mengkarakterisasi pembentukan nanopartikel perak fraksi n-
heksan, etil asetat dan air daun gambir (Uncaria gambir (W. Hunter) Roxb.) dan

untuk mengevaluasi aktivitas toksisitasnya terhadap larva udang Artemia salina.
Fraksinasi dilakukan menggunakan metode cair-cair menggunakan pelarut n-heksan
p.a, etil asetat p.a dan air. Proses sintesis menggunakan metode biosintesis.
Karakterisasi menggunakan alat uji FTIR dan PSA, metode evaluasi dari kategori
tingkat toksisitas berdasarkan nilai LC50 menggunakan metode BSLT. Identifikasi
senyawa dilakukan melalui uji skrining fitokimia dan analisis LC-HRMS, yang
menunjukkan keberadaan berbagai senyawa bioaktif seperti flavonoid, alkaloid,
tannin, saponin, triterpenoid dan steroid. Fraksi etil asetat memiliki kandungan
flavonoid yang tinggi, termasuk katekin, kuersetin dan sianidanol. Hasil
menunjukkan bahwa fraksi etil asetat memiliki ukuran partikel terkecil (246 nm),
zeta potensial tertinggi (+24 mV), PDI (0,42). Vibrasi Ag-O khas AgNPs oksida
pada bilangan gelombang 432,03 dan % transmitansi 54,70 serta toksisitas tertinggi
terhadap larva Artemia salina (LC50=3,309 g/ml) dengan kategori sangat toksik,
diikuti fraksi n-heksan dan fraksi air.

The development of nanotechnology has brought silver nanoparticles (AgNPs),
sized between 1100 nm, to the forefront of medical research. AgNPs are known to
induce cancer cell death through oxidative stress. This study aimed to synthesize and
characterize silver nanoparticles from n-hexane, ethyl acetate, and water fractions of
gambier leaves (Uncaria gambir (W. Hunter) Roxb.) and to evaluate their toxicity
activity against Artemia salina shrimp larvae. Fractionation was carried out using a
liquid-liquid extraction method with n-hexane p.a., ethyl acetate p.a., and water as
solvents. The synthesis process used a biosynthesis method. Characterization was
conducted using FTIR and PSA instruments, while toxicity level evaluation was
based on LC50 values using the BSLT method. Compound identification was
performed through phytochemical screening and LC-HRMS analysis, which showed
the presence of various bioactive compounds such as flavonoids, alkaloids, tannins,
saponins, triterpenoids, and steroids. The ethyl acetate fraction was found to contain
high levels of flavonoids, including catechin, quercetin, and cyanidanol. Results
showed that the ethyl acetate fraction produced the smallest particle size (246 nm),
highest zeta potential (+24 mV), and a PDI of 0.42. The characteristic AgO
vibration of oxidized AgNPs was observed at a wavenumber of 432.03 cm 1 with
54.70% transmittance. It also exhibited the highest toxicity against Artemia salina
larvae (LC50 = 3.309 g/ml), classified as highly toxic, followed by the n-hexane and
water fractions