Search Repository Universitas Fort De Kock

Annona muricata Linn (sirsak) merupakan tumbuhan yang mudah ditemukan digunakan sebagai sumber buah bahkan obat. Masyarakat Indonesia selain mengonsumsi buah sirsak, namun juga memamfaatkan daun sirsak untuk kesehatan dengan cara meminum air rebusan daun sirsak segar. Penelitian ini dilakukan bulan Maret-Agustus 2023 dilaksanakan di Laboratorium Biologi Farmasi UFDK yang bertujuan untuk mengetahui aktivitas antiinflamasi dan antibakteri terhadap Propionibacterium acnes. Metode yang digunakan untuk ekstraksi yaitu maserasi dengan pelarut etanol 70%. Uji skrining fitokimia untuk mengetahui kendungan metabolit sekunder yang terdapat pada sampel. Uji aktivitas antiinflamasi mengunakan metode penghambatan denaturasi protein dengan instrument spektrofotometri Uv-vis konsentrasi 1000 mg/L, 5000 mg/L, 10.000 mg/L dengan nilai IC50 40.946,6. Uji aktivitas antibakteri dilakukan dengan metode difusi agar sumuran konsentrasi 25%, 50%, dan 75% dengan rata rata zona hambat secara berurut sebesar 14,08 mm, 15 mm, dan 17 mm dengan kategori kuat. Dari hasil penelitian dapat disimpulkan bahwa daun sirsak mempunyai potensi aktivitas antiinflamasi dan antibakteri terhadap Propionibacterium acnes.

Annona muricata Linn (soursop) is a readily available plant that serves not only as a source of fruit but also as a medicinal resource. In Indonesia, people not only consume soursop fruit but also utilize soursop leaves for their health benefits by brewing fresh soursop leaf infusions. This research, conducted from March to August 2023 in the Laboratory of Pharmaceutical Biology at UFDK, aimed to investigate the anti-inflammatory and antibacterial activities against Propionibacterium acnes. The extraction method employed was maceration using 70% ethanol as the solvent. Phytochemical screening was carried out to determine the presence of secondary metabolites in the sample. The anti-inflammatory activity was assessed using the denaturation inhibition of proteins with UV-Vis spectrophotometry, and concentrations of 1000 mg/L, 5000 mg/L, 10,000 mg/L were tested, yielding an IC50 value of 40,946.6. The antibacterial activity was evaluated by the agar diffusion method at concentrations of 25%, 50%, and 75%, with respective mean inhibition zone diameters of 14.08 mm, 15 mm, and 17 mm, indicating a strong category of antibacterial activity. Based on the findings, it can be concluded that soursop leaves possess the potential for anti-inflammatory and antibacterial activities against Propionibacterium acnes.