Search Repository Universitas Fort De Kock

Penuaan kulit adalah proses biologis kompleks yangdipengaruhi oleh kombinasi faktor intrinsik, dan ekstrinsik. Faktor Ekstrinsik merupakan factor yang sering kali di abaikan oleh maklukhidup salah satunya yaitu radiasi UVB yang dapat menghasilkanrespons fisiologis. Paparan kulit terhadap UVB dapat menyebabkanphoto-damage yang secara klinis ditandai dengan kekasaran, kerutan, eritema. Penelitian ini menggunakan metode penelitian eksperimental laboratorium (experiment research) menggunakan hewan uji tikusputih jantan galur wistar. Pada awal percobaan, tikus dibagi secaraacak menjadi 7 kelompok, dengan masing-masing kelompok empat ekor dan dilakukan aklimatisasi selama 7 hari. Pada penelitianini, permukaan kulit punggung tikus dicukur dengan mesin cukur dantetap tidak berbulu selama periode percobaan. Tikus di beri paparanradiasi UV-B pada semua kelompok sebesar 50 mJ/cm2 mingguI , 70mJ/cm2 minggu II, dan 80 mJ/cm2 minggu III dan IV dengan durasi paparan 80 detik. Penyinaran diberikan 3x/minggu sehinggadosistotal penyinaran sinar UV-B sebesar 840 mJ/cm2. Pada mingguTerakhir tikus dikorbankan untuk dilakukan pengujian histologi. Hasil penelitian menunjukan Bahwa tidak terdapat perbedaanyang signifikan dari masing-masing formulasi. Berdasarkan hasil rata- rata dari tiap tiap parameter menunjukan formula 2 memiliki rata-ratayang bagus dibandingkan formula lainnya. Kesimpulan dari penelitian ini, Sheet mask sari buah semangkadan lidah buaya memiliki efektifitas terhadap kulit tikus putih Jantanyang di beri paparan radiasi UVB dan diantara semua formula, formula 2 menunjukan hasil yang lebih bagus dibandingkan formulalainnya .

Skin aging is a complex biological process that is influenced by a combination of intrinsic and extrinsic factors. Extrinsic factors are factors that are often ignored by living creatures, one of which is UVB radiation which can produce physiological responses. Skin exposure to UVB can cause photo-damage which is clinically characterized by roughness, wrinkles, erythema. This research uses laboratory experimental research methods using male Wistar white rats as test animals. At the start of the experiment, the mice were randomly divided into 7 groups, with four animals in each group and acclimatized for 7 days. In this study, the surface of the mice's back skin was shaved with a shaving machine and remained hairless throughout the experimental period. Mice were exposed to UV-B radiation in all groups at 50 mJ/cm2 in the first week, 70 mJ/cm2 in the second week, and 80 mJ/cm2 in the third and fourth weeks with an exposure duration of 80 seconds. Irradiation was given 3x/week so that the total dose of UV-B irradiation was 840 mJ/cm2 . In the last week, mice were sacrificed for histology testing. The research results showed that there were no significant differences between each formulation. Based on the average results of each parameter, it shows that formula 2 has a good average compared to the other formulas. The conclusion of this research is that the watermelon and aloe vera juice sheet mask is effective on the skin of male white mice exposed to UVB radiation and among all formulas, formula 2 shows better results than the other formulas.